Next Generation Sequencing Analysis

MAJ-NGSA (Next Generation Sequencing Analysis)

High-throughput sequencing technologies are continuously being developed and upgraded to provide cost-effective, Time saving and parallelization, thus producing millions of reads simultaneously. Therefore, biomedical research projects are heavily relying on NGS for a variety of genome-wide scale studies including DNA-seq, RNA-seq, exome-seq, ChIP-Seq, DNA-Methylation, and many more in order to study genomic and transcriptomic profiles, DNA-protein interactions and epigenomic characterization. Through MAJ-NGSA, we help Biotech companies, academic institutions and private research labs in the computational analysis of High-throughput NGS data. Our NGS analysis service starts from connecting the researcher to the best NGS providers to customized downstream analyses, biological interpretation and visualization.



  • Alignment against reference genome to against regions of interest.
  • Sample Quality control
  • Variant calling.
  • Variant annotation.
  • Identification of structural variants.
  • Copy Number Variant (CNV) analysis.
  • Genome Annotation.
  • Metagenomic and Microbiome analysis.


  • Identification of genes and protein-coding regions.
  • Quality control with FASTQC
  • Transcriptome and Genome Aignment.
  • Normalization and Transformation
  • Differential Expression at gene level and across isoforms.
  • Alternative Splicing Analysis.
  • Pathway Analysis.
  • Transcript Quantification


DNA-Methylation Sequencing

  • Alignment using Bismark, a methylation-specific aligner.
  • DNA methylation measurements with the 450K array.
  • Quantification of CpG island methylation.
  • Differentially methylated Regions (DMRs) Analysis.
  • Functional Annotation of DMRs.
  • Whole Genome analysis



  • Peak and Motif detection.
  • Quality control.
  • Annotation of DNA-protein interactions.