124 Othman Ibn Affan, Al Matar, Qism El-Nozha, Cairo Governorate 11361
+2 010 9001 3588
info@majecules.com

MAJ-NGSA (Next Generation Sequencing Analysis)

High-throughput sequencing technologies are continuously being developed and upgraded to provide cost-effective, Time saving and parallelization, thus producing millions of reads simultaneously. Therefore, biomedical research projects are heavily relying on NGS for a variety of genome-wide scale studies including DNA-seq, RNA-seq, exome-seq, ChIP-Seq, DNA-Methylation, and many more in order to study genomic and transcriptomic profiles, DNA-protein interactions and epigenomic characterization. Through MAJ-NGSA, we help Biotech companies, academic institutions and private research labs in the computational analysis of High-throughput NGS data. Our NGS analysis service starts from connecting the researcher to the best NGS providers to customized downstream analyses, biological interpretation and visualization.

DNA-Seq

  • Alignment against reference genome to against regions of interest
  • Sample Quality control
  • Variant calling
  • Variant annotation
  • Identification of structural variants
  • Copy Number Variant (CNV) analysis
  • Genome Annotation
  • Metagenomic and Microbiome analysis

RNA-Seq

  • Identification of genes and protein-coding regions
  • Quality control with FASTQC
  • Transcriptome and Genome Aignment
  • Normalization and Transformation
  • Differential Expression at gene level and across isoforms
  • Alternative Splicing Analysis
  • Pathway Analysis
  • Transcript Quantification

DNA-Methylation Sequencing

  • Alignment using Bismark, a methylation-specific aligner
  • DNA methylation measurements with the 450K array
  • Quantification of CpG island methylation
  • Differentially methylated Regions (DMRs) Analysis
  • Functional Annotation of DMRs
  • Whole Genome analysis

ChIP-Seq

  • Peak and Motif detection
  • Quality control
  • Annotation of DNA-protein interactions